Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
R Holland Cheng and Mo A Baikoghli
UC Davis, USA
Scientific Tracks Abstracts: J Proteomics Bioinform
Cryogenic electron microscopy (cryo-EM) provides a visible and quantitative approach to investigate the function of structural proteins of viruses, both in assembly and disassembly. The fundamental biochemical properties of metastable intermediates and stable mature form of viral capsids, responsible for their capacity to resist extreme pH and digestive enzyme degradation, are revealed through cryo-EM. In this presentation, the structural functionality of Hepatitis E viral nanoparticles (HEVNP), as a multimodal delivery platform, is assessed for the delivery of medicals or agents to target tumors through both circulation and mucosal routes. Aided by multimodal imaging and integrative machine learning networks, the targeting, tissue distribution, and payload packaging are investigated and demonstrated. Together, cryo-EM and cellular tomography are exemplified in the recent outcomes of unveiling the molecular mechanisms essential for platform vector design towards the success of constructing a non-invasive, mucosal targeting system.