Journal of Fundamentals of Renewable Energy and Applications

Journal of Fundamentals of Renewable Energy and Applications
Open Access

ISSN: 2090-4541

+44 1300 500008

Cyanobacteria isolated from Amazonian rivers growing in vinasse for biodiesel production


2nd International Congress and Expo on Biofuels & Bioenergy

August 29-31, 2016 Sao Paulo, Brazil

Wallace Rafael de Souza, Diego Bonaldo Genuario, Itamar Soares Melo, Marcia Maria Parma and Regina Teresa Rosim Monteiro

University of S�£o Paulo, Brazil
Embrapa Environment, Brazil

Posters & Accepted Abstracts: J Fundam Renewable Energy App

Abstract :

Cyanobacteria is a very versatile group of microorganisms thriving in the most extreme conditions. Its potential as a source of energy is attributed to its fast growth and high photosynthetic rates. However, information regarding its diversity in some environments is still scarce. Notwithstanding, the selection of suitable strains and use of residues from the industry can contribute to costs reduction in a large-scale system. In this context, the use of sugarcane vinasse, the main residue of ethanol production, to obtain biomass from Cyanobacteria represents a great deal. Twelve nonaxenic Cyanobacterial strains isolated from Amazon and Solim�µes were tested in three culture media added with vinasse (1%, 2.5%, 5% and 10% v/v) and ultrapure water (2.5%, 5% and 10% v/v) in 24-well plates at room temperature. These isolates were morphologically and phylogenetically characterized using the 16S rRNA gene sequence. Eleven strains grew in at least one concentration of media+vinasse and two in water+vinasse (pH varing from 4.5 to 6.7). Visually, increasing the vinasse concentration caused biomass reduction and proliferation of residual yeast (optical microscopy). In most isolates, the fatty acids profile changed accordingly to their types and amounts with regard to those used for biodiesel production. Considering the unicellular Chroococcidiopsis sp (# 20) in BG-110 medium with 5% vinasse, the fatty acid C16:1 decreased from 17.46% to 11.81% and C18:1 increased from none to 24.79% when compared to BG-110 medium. These results suggest that cyanobacteria strains from Amazonian region tolerate vinasse until 5% without any pH adjust with undesired yeast proliferation.

Biography :

Wallace Rafael de Souza graduated in Environmental Engineering from Faculdade de Jaguariúna/Sligo Institute of Technology (2015) and has experience in environmental microbiology (bioprosprecting and biotechnology of microorganisms and taxonomy of actinomycetes). Currently, he is doing his Master’s degree in Science at University of São Paulo (CENA-USP) with potential of Cyanobacteria from rivers of the Amazon region for biodiesel production. He has published 6 papers in peer-reviewed journals.

Email: walla_souza@hotmail.com

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