Journal of Proteomics & Bioinformatics
Open Access

Design and characterization of antimicrobial peptides with high anticancer activity and selectivity

International Conference on Protein Engineering

October 26-28, 2015 Chicago, USA

Jya-Wei Cheng1, Hung-Lun Chu1, Bak-Sau Yip2, Hui-Yuan Yu1, Hsi-Tsung Cheng1, Ya-Han Chih1 and Kuan-Hao Chen1

1National Tsing Hua University, Taiwan 2National Taiwan University Hospital Hsin-Chu Branch, Taiwan

Posters-Accepted Abstracts: J Proteomics Bioinform

Abstract :

We describe a strategy to boost anticancer activity and reduce normal cell toxicity of short antimicrobial peptides by adding positive charge amino acids and non-nature bulky amino acid �²-naphthylalanine residues to their terminal. The designed peptide displayed salt resistance and small toxicity to hRBCs and human fibroblast. Fluorescence microscopic studies indicated that the FITC-labeled peptide preferentially binds cancer cells and causes apoptotic cell death. Moreover, a significant inhibition in human lung tumor growth was observed in the xenograft mice treated with designed peptide. Our strategy provides new opportunities in the development of highly effective and selective antimicrobial and anticancer peptide-based therapeutics.

Biography :

Email: jwcheng@life.nthu.edu.tw