Journal of Chromatography & Separation Techniques
Open Access

Determination of niacinamide in cereal, vitamin supplements and cosmetics by HPLC: How the sample affects the required sample preparation

World Congress on Chromatography

September 21-23, 2016 Amsterdam, Netherlands

Karyn M Usher, Daniel Keating and Henry F Rossi III, Michael J Bozym, Anna Glinko and Michelle L Owens

West Chester University, USA

Posters & Accepted Abstracts: J Chromatogr Sep Tech

Abstract :

Chromatography is a technique used for the separation of mixtures. This is a great simplification of the practical application of chromatography to the separation, identification and quantification of analytes of interest in complex matrices. Sample matrices often contain interferences, thereby producing complex chromatograms in which the analyte of interest is difficult to determine. This presentation describes sample preparation involved in the analysis of niacinamide in complex matrices including beauty products, vitamin supplements and breakfast cereal. Niacinamide is often included in vitamin supplement tablets, and drinks. These samples typically require only minimal sample preparation. This section will include a discussion about common errors to avoid in sample preparation which are important even for simple methods. Niacinamide is also a common additive in personal care products. It�s often used as a whitening agent in lotions to lighten skin and give more even complexion. Lotions and creams containing niacinamide are widely used around the world for both medical and aesthetic reasons. Many products list niacinamide in the ingredients but do not give the concentration used. In this study, the amount of niacinamide in several Olay products was determined using Liquid-Liquid Extraction and HPLC. Breakfast cereals are often fortified with niacinamide because it is necessary for proper body function. These foods may contain structurally/chemically similar compounds such as nicotinic acid, vitamers and precursors. This typically means that more involved sample preparation is necessary. In this study, the amount of niacinamide in a cereal was determined by solid phase extraction and HPLC.

Biography :

Email: karyn.usher@gmail.com