ISSN: 2090-4541
+44 1300 500008
Steffy and Naveen Gupta
Panjab University, India
Posters & Accepted Abstracts: J Fundam Renewable Energy Appl
Bioprocessing of pulps using microbial enzymes for biobleaching is one of the most suitable biological applications
for the pulp and paper industry. Lignolytic (laccase,) enzymes helps directly in the removal of lignin from the
pulp and hemicellulolytic (xylanase, mannanase) enzymes increases the access of bleaching chemicals to the lignin
layer by opening the pulp structure. Bacterial enzymes are preferred as they can work at high temperature and pH at
which the pulp is processed in industry. A number of these enzymes from bacteria have been explored individually
for pulp biobleaching. Some have also reported the use of combination of these enzymes but produced by different
bacteria which make their application economically unviable. Therefore, there is a need to isolate a bacterial strain
producing a cocktail of hemicellulolytic and lignolytic enzymes and standardizing a procedure for their application
in pulp and paper industry to make the whole process of paper making eco- friendly. In present study, Bacillus sp.
LXM 55 MTCC 12897 was isolated from effluent of paper mill which produced a cocktail of lignolytic (Laccase)
and hemicellulotic (Xylanase+Mannanase). All the enzymes were found to be active in the temperature and pH
range which was suitable for their application in pulp and paper industry. Conditions were standardized for the over
production of enzymes in solid state fermentation by using classical and statistical methods. Significant reduction in
chlorine use was achieved by pretreating the pulp with cocktail of enzymes. Therefore, this cocktail of enzymes is a
highly suitable candidate for developing a cleaner and economical process for pulp bleaching.
Recent Publications:
1. Bajpai P, Anand A, Sharma N, Mishra S P, Bajpai P K and Lachenal D (2006) Enzymes improve ECF
bleaching of pulp. Bioresources 1:34-44.
2. Chauhan P S, Soni S K, Sharma P, Saini A and Gupta N (2014) A Mannanase from Bacillus nealsonii PN-
11: statistical optimization of production and application in biobleaching of pulp in combination with
xyalanse. International Journal of Pharma and Bio Sciences 5:237-251.
3. Gupta V, Garg S, Capalash N, Gupta N and Sharma P (2015) Production of thermo-alkali-stable laccase
and xylanase by co-culturing of Bacillus sp., and B. halodurans for biobleaching of kraft pulp and deinking
of waste paper. Bioprocess and Biosystem Engineering 38:947-956.
4. Sharma P, Sood C, Singh G and Capalash N (2015) An eco-friendly process for biobleaching of eucalyptus
kraft pulp with xylanase producing Bacillus halodurans. Journal of Cleaner Production 87:966-970.
5. Sondhi S, Sharma P, George N, Chauhan P S, Puri N and Gupta N (2015) An extracellular thermo-alkalistable
laccase from Bacillus tequilensis SN4, with a potential to biobleach softwood pulp. 3 Biotech.
3(5):175-185.
Steffy is a PhD Research Scholar in Department of Microbiology at Panjab University, Chandigarh, India. She has received her Bachelor Degree in Biotechnology from Doaba College, Guru Nanak Dev University, India. She holds a Master’s Degree in Microbiology that focuses on isolation and screening of silica solubilizing thermophilic fungi for enhancing paddy straw digestibility and biogas production. Her current Doctoral research is based on isolation of a bacterium producing cocktail of lignolytic and hemicellulolytic enzymes and its application on pulp and paper industry. She is keen to spread her knowledge in the field of biobleaching in pulp and paper industry in an economical and eco-friendly manner and provide green technology in near future.
E-mail: steffyangural91@gmail.com