Immunome Research
Open Access
ISSN: 1745-7580
+44-77-2385-9429
ISSN: 1745-7580
+44-77-2385-9429
Dapeng Peng, Wei Han and Zonghui Yuan
Huazhong Agricultural University, China
Posters & Accepted Abstracts: Immunome Res
Nitroimidazoles (NDZs) are a well-known group of antiprotozoal and bactericidal agents in medicine and veterinary medicine. They are also used for the growth promotion and improvement of feed efficiency in the livestock industry. However, the misuse of nitroimidazoles (NDZs) can lead to NDZs residues in edible animal tissues, which would be harmful to consumer health. To quickly monitor NDZs residues in edible animal tissues and feed, a monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) with a simple sample preparation method and clean-up was developed in the present study. At first, a broad-specificity monoclonal antibody, 1D5, against NDZs has been produced, which the IC50 values of the NDZs, dimetridazole, ipronidazole, ronidazole hydroxydimetridazole and hydroxyipronidazole were 4.79 �¼g L-1, 0.47 �¼g L-1, 5.97 �¼g L-1, 23.48 �¼g L-1 and 15.03 �¼g L-1, respectively. The limit of detection of the method for the NDZ matrix calibration ranged from 4.2-50.3 �¼g kg-1 in the feed matrices and from 0.11-4.11 �¼g kg-1 in the edible animal tissues matrices. The recoveries of the NDZs were in the range of 75.5-111.8%. The CVs were less than 14.4%. A good correlation (r=0.9905) between the ELISA and HPLC-MS results of the tissues demonstrated the reliability of the developed IC-ELISA. In a word, this simple method reduced the time required for sample preparation, ensured greater throughput and met the requirements for nitroimidazoles residues analyses. It can be used as a useful tool for screening of NDZs in animal edible tissue and feed.
Email: pengdapeng@mail.hzau.edu.cn