Journal of Chromatography & Separation Techniques
Open Access

Development of an UPLC-MS based method for the simultaneous quantitation of phenolic components in honey using multi-walled carbon nanotubes as solid phase adsorbents

World Congress on Chromatography

September 21-23, 2016 Amsterdam, Netherlands

Zeid Abdullah Alothman

King Saud University, Saudi Arabia

Posters & Accepted Abstracts: J Chromatogr Sep Tech

Abstract :

An UPLC-MS method has been developed for the simultaneous separation, identification and determination of 22 phenolic constituents in honey from various floral sources from Yemen. Solid-phase extraction was used for extraction of the target phenolic constituents from honey samples, while multi-walled carbon nanotubes were used as solid phase adsorbent. The chromatographic separation of all phenolic constituents was performed on a BEH C18 column using a linear gradient elution with a binary mobile phase mixture of aqueous 0.1% formic acid and methanol. The quantitation was carried out in selected ion reaction monitoring acquisition mode. The total amount of phenolic acids, flavonoids and other phenols in each analyzed honey was found in the range of 338-3312, 122-5482, and 2.4-1342 �¼g/100 g of honey, respectively. 4-hydroxybenzoic acid was found to be the major phenolic acid. The main detected flavonoid was chrysin, while cinnamic acid was found to be the major other phenol compound. The regeneration of solid phase adsorbent to be reused and recovery results confirm that the proposed method could be potentially used for the routine analysis of phenolic constituents in honey extract.

Biography :

Email: zaothman@ksu.edu.sa