Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Zhang Pi-hong
Accepted Abstracts: J Proteomics Bioinform
In this study, polymorphorphonuclear neutrophils (PMN) transmigration followed adhesive via Human umbilical vein endothelial cells stimulated with burn serum and retention in burn blister fluid model were established using transwellsTM inserts. PMN were harvested from healthy volunteers, burn serum was isolated from massive burn patients within 48 hrs after burn injury, and burn blister fluid was collected from intact blisters on burn wound within 24 post burn hours. The total proteins of PMN in healthy volunteers (A group), transmigration followed adhesive (B group), retention in burn blister fluid (C group) were separated by immobilized pH gradient-based two-dimensional polyacrylamide gel electrophoresis(2-DE), and the 2-DE images were analyzed by PDQuest 7.1.0 software. Discrepant expression protein spots were analyzed by Matrix-assisted laser desorption/ ionization time of flight mass spectrometry(MALDI-TOF-MS) and peptide mass fingerprinting obtained and searched in database to identify of the proteins. 33 differentially expressed proteins spot were found between normal PMN group and the others groups, and 22 spots (16 proteins) were identified, including: Profilin-1, Actin, Heat shock 70 kDa protein, Peptidyl-prolyl cistrans isomerase A, Leukocyte elastase inhibitor, Fructose-bisphosphate aldolase A, Catalase, Alpha-enolase, Vimentin, Tubulin beta chain, Lactotransferrin, Chloride intracellular channel protein 1, Glyceraldehyde-3-phosphate dehydrogenase, Protein DJ- 1, Protein S100-A9 and F-actin-capping protein subunit alpha-1.These proteins involve in the inflammation amplification and immune disorder, and play an important role in capillary permeability and PMN apoptosis delay
Zhang Pi-hong has completed his Ph.D at the age of 30 years from Hunan Medical University and serious proteomic studies of PMN on burn sepsis sponsored by Natural Science Foundation of China. He received a Microsurgery Training in Alfred Hospital (Melbourne, Australia) ten years ago, and now he is a professor of Central South University. He has published more than 20 papers in reputed journals and serving as a member of Chinese burn association.