Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
+44 1300 500008
ISSN: 2157-7064
+44 1300 500008
Junqing Zhang
Hainan Medical University, China
Posters & Accepted Abstracts: J Chromatogr Sep Tech
Galangin (3,5,7-trihydroxyflavone) is present in high concentrations in Alpinia officinarum Hance and it shows multifaceted in vitro and in vivo biological activities. The number and position of hydroxyl groups in this molecule play an important role in these biological activities. In order to systemic clarify the exposure of galangin after oral and intravenous administration to rats, two LC-MS/MS methods were developed and validated and successfully applied to analyze the parent drug molecules and aglycones liberated from plasma samples via �²-glucuronidase hydrolysis. Our major findings were as follows: (1) The routes of administration showed significant influences on the systemic exposure of galangin and its metabolites; (2) Galangin was preferentially glucuronidated after p.o. dosing but sulfated after i.v. medication; (3) Kaempferol conjugates were detected demonstrating that oxidation reaction occurred; however, both glucuronidation and sulfation were more efficient; and (4) Oral bioavailability of free parent galangin was very low. The results showed that systemic exposure to galangin and its metabolites was different in rat plasma between oral and intravenous administration. Further research is needed to characterize the structures of galangin conjugates and to evaluate the biological activities of these metabolites.
Email: jqzhang2011@163.com