Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Sunil Pandey
Pokhara University, Nepal
Posters & Accepted Abstracts: J Proteomics Bioinform
Among the many infectious diseases, tuberculosis (TB) remains the worldâ��s leading cause of death. Worldwide, 37% of new cases went undiagnosed or were not reported. There are very few reports on direct detection of rpoB & katG gene mutations in Nepal. The vast majority of Rifampicin (RIF)-resistant M. tuberculosis clinical isolates have mutations in the gene rpoB, which encodes the �²-subunit of RNA polymerase. However, most of the INH resistance mutations occurred in codon 315 and this was in agreement with other mentioned studies showing the major involvement of this codon in INH resistance all over the world. Most scrutinize literature was collected from different sources including PubMed, HINARI. 45 samples were collected from Annapurna Neurological Institute of Allied Sciences, Kathmandu Nepal and study was carried out in Decode Genomics and Research Center Pvt. Ltd Sinamangal, Kathmandu, Nepal. Among collected sample 27 were from male age ranged from 19-71 and 18 were female patients, age ranged from 22-71 years. Collected sample was first decontaminated by Petroffs modified method and following DNA was extracted. Out of 45, 6 samples were found to be AFB positive by ZN method-PCR was found to be positive for 31(68.88%) samples and 14 (31.11%) samples were found negative. Furthermore, rpoB mutation was found in 3 patients (6.66%) and katG gene mutation was also found in 3 patients (6.66%) of total sample. One sample shows both katG and rpoB gene mutation. We are now come to comprehend that from direct clinical samples mutation can be detected, this is even reliable when result need to disseminate fast for treatment. However, large sample size is needed to validate these findings.