Clinical & Experimental Cardiology
Open Access
ISSN: 2155-9880
+44 1300 500008
ISSN: 2155-9880
+44 1300 500008
Nitin G Dumore and Monali N Dumore
Dadasaheb Balpande College of Pharmacy, India
Posters & Accepted Abstracts: J Clin Exp Cardiolog
Objective: Atherothrombosis is a disorder which may lead to increased incidence of cardiovascular diseases. One of the causes for this disorder is platelets aggregation and adhesion. The objective of the present study is to study effect of resveratrol (resv) and clopidogrel (clopi) individually and in combination with inhibition of platelet aggregation and adhesion by in vitro and in vivo method. Material & Method: The platelet aggregation was measured by whole blood aggregometer (Chrono-Log Corporation) in 0.5 ml sample of PRP mixed with ADP by measuring infrared light transmission using different concentration of resveratrol and clopidogrel individually and in combination. Platelet adhesion was measured by micro-plate reader, lab system (Merck Pvt. Ltd.). Nitric oxide estimation was done by nitric oxide colorimetric estimation kit with retro orbital method using healthy male Swiss albino rats. Result: Resveratrol and clopidogrel at the effective concentration produced minimal platelet aggregation and adhesion effect, when combined together didn�t show any synergistic or additive effect. There was no effect of resveratrol on clopidogrel or vice versa on their inhibitory effect. Both individually and in combination, increased bioavailability of nitric oxide revealed significant P<0.05. Conclusion: Since patients on antiplatelet therapy may consume beverages like grape juice, red wine, peanuts etc. which are rich sources of resveratrol do not show any synergistic or additive effect on platelet function, but, increased bioavailability of nitric oxide lead to adverse outcome.
Email: nitingdumore@gmail.com