Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
David Sheehan, Arjun Prakash Sridharan, Hugh Doyle and Yao Zhu
University College Cork, Ireland
Posters & Accepted Abstracts: J Proteomics Bioinform
Nanomaterials are an important category of emerging environmental threat given that they are being used in ever-increasing amounts and for an ever-wider range of applications. Nanoparticles, a sub-set of nanomaterials, are particles of average diameter < 100 nm and are readily prepared from metal oxides. Metal oxide nanoparticles are often used in sun creams and other topical preparations. In this study the sensitivity of a panel of human cell lines (HEk, HeLa and A172) to TiO2, CuO2 and ZnO2 nanoparticles was assessed. Cytotoxicity was determined using both neutral red retention and the MTT assays. These suggested that cells had much lower LC50 with ZnO2 and CuO2 than with TiO2 and that both assays gave comparable results. A more detailed study was made of CuO2 effects on HeLa cells using redox proteomics. Thiol-containing proteins were labelled with 5-iodoacetamido-fluorescein (IAF) and fluorescence images were obtained in a Typhoon scanner. Gels were then stained with colloidal coomassie and all images were analysed with SameSpots image analysis software. Differentially expressed and selectively oxidised proteins were identified by peptide mass fingerprinting by LC-tandem MS. Our data suggested that >20 individual proteins are selectively oxidised in response to CuO2.
Email: d.sheehan@ucc.ie