Virology & Mycology
Open Access
ISSN: 2161-0517
ISSN: 2161-0517
Hanna Radecka and Jerzy Radecki
Posters-Accepted Abstracts: Virol-mycol
In recent years much attention in virology has been focused on developing of affordable and adequate methods for detection of whole viruses or their fragments. We have developed of several types of immunosensors as well as genosensors destined for the detection of Highly-Pathogenic Avian Influenza type H5N1 virus (HPAI) spreading among wild and domestic birds. The immunosensor were developed by the successive modification of gold as well as glassy carbon electrodes. The whole antibody or their fragments have been applied as the sensing elements. The complex between virions and specific antibody adsorbing on a surface of an electrode forms an insulating layer. This phenomenon, which is a base of ion-channel mimetic type of immunosensors can be monitored by the electrochemical impedance spectroscopy (EIS) in the presence of redox marker. The other type of immunosensors are based on redox active layers incorporated di-pyrromethene-Cu (II) or phenanthroline-Epoxy- Fe (III) complexes. The changes of electrochemical parameters of redox centres upon target analyte binding are the base of analytical signal generation. The both type of immunosensors displayed in order of magnitude better sensitivity in comparison to ELISA. They are also very selective. The matrix from hen sera has no influence on the immunosensors performance. To face of the need of systems for simultaneous determination of few markers of one disease coming from medical diagnosis, we have developed a novel dual DNA electrochemical sensor with â??signal-offâ?? and â??signal-onâ?? architecture for simultaneous detection of two different sequences of DNA derived from Avian Influenza Virus type H5N1 by means of one electrode. Two sequences of ssDNA characteristic for hemagglutinin decorated with ferrocen and characteristic for neuraminidase decorated with metylene blue were immobilized covalently together on the surface of one gold electrode. The detection limit in the fM range has been achieved with genosensors incorporated with ssDNA decorated with Co (II)-porphyrin as well as with 3-irin bis (dicarbiollide).The immunosenosors as well as genosensors could be recommended for the direct electrochemical detection of viruses as well as antibodies in the natural physiological samples.