Biochemistry & Pharmacology: Open Access
Open Access
ISSN: 2167-0501
+44-77-2385-9429
ISSN: 2167-0501
+44-77-2385-9429
Yuzhu Zhang and Hongfeng Chen
Longhua Hospital Affiliated to Shanghai University of TCM, China
Scientific Tracks Abstracts: Biochem Pharmacol (Los Angel)
MDA-MB-231/DDP had higher IC50 value of DDP, lower intracellular DDP concentration, lower apoptosis ratio than MDAMB- 231 cell line treated with DDP. Considering the intracellular DDP concentration difference, we tested drug-resistant membrane proteins (MRP2, P-gp and BCRP), which were remarkably increased in MDA-MB-231/DDP cells. Next, we found these increased membrane proteins were induced by the activation of NF-�ºB pathway in MDA-MB-231/DDP cells. Furthermore, ETS1, RPL6, RBBP8, BIRC2, PIK3A and RARS were six important genes for DDP-resistance based on PPI network and expression validation. However, it has been reported enforced over-expression of ETS1 induced IKK�± mRNA and protein expression as well as IKK�± promoter activity. Our results suggested the protein expression of ETS1 and IKK�± were significantly up-regulated in MDAMB- 231/DDP cells. In addition, inhibition of ETS1 expression enhances chemo-sensitivity to DDP and reversed the activation of NF- �ºB pathway in MDA-MB-231/DDP cells, whether ETS-1 binds to the core IKK�± promoter and strongly induces its activity. Now, our team is researching the corresponding binding sites between ETS1 and IKK�±by dual-luciferase and chromatin immunoprecipitation technique (ChIP).
He is studying his PhD of medicine at Shanghai University of Traditional Chinese Medicine. His researches focus on key target genes of tumor prognosis, mechanisms of drug resistance and anti-cancer natural drugs.