Immunome Research
Open Access
ISSN: 1745-7580
+44-77-2385-9429
ISSN: 1745-7580
+44-77-2385-9429
Yewubdar Gulelat, Ketema Tafess, Aboma Zewde, Mulualem Ambaw, Markos Abebe, and Gobena Ameni
Dilla University, Ethiopia
Armauer Hansen Research Institute, Ethiopia
Jimma University, Ethiopia
Addis Ababa University, Ethiopia
Posters & Accepted Abstracts: J Immunome Res
The control of bovine tuberculosis is difficult in developing countries because of the high cost of a sustainable testing programs and the lack of a diagnostic assays with high sensitivity and specificity to detect animals at all stages of infection. A cross-sectional study was conducted to evaluate the test agreement between comparative intra-dermal tuberculin test (CIDT) and interferon gamma (INF-�³) tests for the diagnosis of bovine tuberculosis (BTB) at Holeta, Ethiopia from December, 2012 to May, 2013. In this study, a total of 114 cattles were tested both by CIDT and INF-�³ tests. The agreement between INF-�³ (PPDB-PPDA) and CIDT tests was fair (k=0.288). On the other hand, a moderate agreement (k=0.44) was observed between INF-�³ test (PPDB-PPDA) and INF-�³ test (ESAT6/CFP10-Nil). The difference between reaction sizes at the bovine PPD injection site and the avian PPD injection site was significant (t test; P<0.001). While there was no significance difference (t test; P>0.05) in INF-�³ response to bovine PPD and avian PPD. INF-�³ response was stronger for ESAT6-CFP10 protein cocktails compared to PPD (B-A). Logistic regression analysis showed a significant association of animal age group with the responses of CIDT and INF�³ tests. In conclusion, the fair agreement between the CIDT and INF-�³ tests could indicate that the two tests are not completely overlapping and thus in addition to detecting a similar stage of the disease they also detect the different stages of the disease. Hence, it is advisable to use the two tests in combination.
Email: yewub2@yahoo.com