Journal of Glycobiology
Open Access

Extracellular matrix as an adhesion promoter

Glycobiology World Congress

August 10-12, 2015 Philadelphia, USA

Samira Bagheri

Posters-Accepted Abstracts: J Glycobiol

Abstract :

Carbohydrates are the main component of the extracellular matrix (ECM) where they associate with proteins to form
glycoproteins or proteoglycans or exist as long-chain disaccharides. All ECM proteins except elastin have associated sugar
and in some cases, ECM proteins require proper glycosylation to achieve the full biological activity. It is also now clear that
many ECM proteins have carbohydrate-binding domains that specifically recognize and interact with glycoconjugates with
other matrix components and on the cell surface. Carbohydrates have been implicated in a wide variety of processes ranging
from cell adhesion and migration to matrix assembly, growth factor sequestration and regulation, involvement in many aspects
of immune function, binding of plasma proteins and control of thrombogenesis. This contribution is a method of immobilizing
and processing functional multi-component structures of the ECM comprising the following successive process steps including
covalent binding of an adhesion promoter layer to cell culture carriers culturing cells of a desired type on the adhesion promoter
layer and thus immobilizing the ECM secreted by the cells by secretion and binding to the adhesion promoter layer and
application of a decellularization protocol so as to detach matrix secreting cells from the surface while simultaneously retaining
the structure and functionality of the immobilized ECM which is connected to the adhesion promoter.