Journal of Biomedical Engineering and Medical Devices
Open Access
ISSN: 2475-7586
ISSN: 2475-7586
Patrick Rhodius
Harvard Medical School, USA
Posters & Accepted Abstracts: J Biomed Eng Med Devic
This study investigated the potential of uncultured-Stromal-Vascular-Fraction (SVF) cells in promoting facial nerve regeneration in a rat model. A 7 mm nerve defect was created in the buccal branch of facial nerve in five groups of Lewis rats (total n=30, n=6 per group). A silicone tube, infused with three types of solutions with different syngeneic uncultured SVF cell numbers; 1Ã?Â?103 cells (1Ã?Â?103 cell group, n=6), 1Ã?Â?105 cells (1Ã?Â?105 cell group, n=6) and 1Ã?Â?107 cells (1Ã?Â?107 cell group, n=6) was implanted into the facial nerve defect. The silicone tube transplantation was performed by the previously reported two-point mattress suture method, a 1 mm length of nerve stump being inserted into the tube by pulling both sutures. Nerves were examined at 13 weeks after the surgery. The findings were compared to the autograft and collagen-alone groups with Facial Palsy Score (FPS), the number of myelinated fibers, fiber diameter, axon diameter, myelin thickness and g ratio. There was no significant difference in FPS between autograft and 1Ã?Â?105 cell groups at 13 weeks after surgery and FPS values of these two groups were significantly higher than those of the other three groups (P<0.01). Axon diameter significantly increased in the 1Ã?Â?105 cell group compared with the 1Ã?Â?103 (P<0.05) and 1Ã?Â?107 cell groups (p<0.01). Myelin thickness was found to be the highest in the autograft group followed by the 1Ã?Â?105, 1Ã?Â?103, 1Ã?Â?107 cell and negative control groups and there were significantly differences among all groups (P<0.01). The infusion of uncultured-SVF into the artificial nerve conduit promoted optimal nerve regeneration that was significantly better than nerve conduit alone. References 1.Watanabe, et al. (2017) Undifferentiated and differentiated adipose-derived stem cells improve nerve regeneration in a rat model of facial nerve defect. J Tissue Eng Regen Med; 11(2): 362-374. 2.Yorikatsu, et al. (2016) Dual innervation method using one-stage reconstruction with free latissimus dorsi muscle transfer for re-animation of established facial paralysis: simultaneous reinnervation of the ipsilateral masseter motor nerve and the contralateral facial nerve to improve the quality of smile and emotional facial expressions. Journal of Plastic, Reconstructive & Aesthetic Surgery; 62(12): 1589-1597. 3.Iverson Ronald, et al. (2015) MOC-PS (SM) CME Article: Liposuction. Plastic and Reconstructive Surgery; 121(4): 1-11. 4.Matsumine, et al. (2011) Surgical procedure for transplanting artificial nerve conduits for peripheral nerve regeneration. Plastic and reconstructive surgery; 128(2): 95e-97e. 5.Sasaki, et al. (2014) Electrophysiologic and Functional Evaluations of Regenerated Facial Nerve Defects with a Tube Containing Dental Pulp Cells in Rats. Plastic and Reconstructive Surgery; 134(5): 970-978.
Patrick Rhodius is presently working at Harvard Medical School, Boston, where he continues his experimental research at the HMS Tissue Engineering and Wound Healing Laboratory. He is routinely into clinical and surgical procedures, assisting a broad spectrum of peripheral-nerve microsurgery procedures including hand surgery and reconstructive facial surgery operations. He is also the integral Biomedical Engineering Specialist of the HMS lipografting studies team, playing a vital role from the beginning of a study to its conclusion.