Orthopedic & Muscular System: Current Research
Open Access

Far infrared emitted by ceramic materials increases paw temperature and reduces mechanical hypersensitivity and knee edema in a rat model of monoiodoacetate-induced Osteoarthritis

3^rd International Conference and Exhibition on Orthopedics & Rheumatology

July 28-30, 2014 DoubleTree by Hilton Hotel San Francisco Airport, USA

Francisco J Cidral-Filho, Ana Paula Batisti, Diogo Cunha dos Reis, Antônio Renato Pereira Moro, Anna Paula Piovezan and Daniel Fernandes Martins

Accepted Abstracts: Orthop Muscul Syst

Abstract :

Objective: This study investigated the effect of far infrared emitted by ceramic materials on skin temperature, paw mechanical hypersensitivity and knee edema in a rat model of monoiodoacetate (MIA)-induced osteoarthritis. Methods: Experiments were conducted with male Winsar rats (200-250 g) anesthetized with a mixture of ketamine and xylazine (50 and 10 mg/kg, respectively, i.p.). Joint damage was induced by a single intra-articular injection of MIA (1 mg/50 μl; Sigma UK - which disrupts glycolysis resulting in chondrocyte death) through the infrapatellar ligament of the right knee. Control animals received a single injection of saline (50 μl). Three separate measures were assessed: (1) Thermal analyses of the central areas of the front paws of the animals (with a portableThermaCAM® E320 infrared camera - Flir, Sweden - with a 320×240 pixels resolution, thermal sensitivity of <0.10°C at 25°C and accuracy of ±2°C - positioned 0.5 m away from the animals paws. The infrared images were analyzed with the FLIR QuickReport 1.2 software); (2) hindpaw mechanical withdrawal thresholds (using von Frey monofilaments - Semmes-Weinstein monofilaments of bending forces 1-15 g), which provide an index of central sensitisation; and (3) edema formation (measured with a digital caliper - Pantec, Brazil), which is directly associated with the localized inflammatory response. For treatment a BiopowerTM ceramics Pad (80% BioCorn PVC - 20% ceramic materials) was placed inside the animals box; control animals were placed on a Sham Pad (100% BioCorn PVC without ceramics) and underwent the same experimental protocol. Results: On day 3 post-MIA injection acute exposure (2 hours) to the BioPower TM ceramics Pad increased paw temperature (±4ºC), although only chronic exposure to the treatment (Day 7 and10 post-MIA) reduced mechanical hypersensitivity (p<0.001) and knee edema (p<0.001). Conclusion: Far infrared emitted by ceramic materials increased paw temperature (after acute exposure) whereas only prolonged treatment reduced mechanical hypersensitivity and knee edema in a rat model of MIA-induced osteoarthritis