Organic Chemistry: Current Research
Open Access

Generation of droplets of liquid for mass spectrometry

2^nd International Conference on Past and Present Research Systems of Green Chemistry

September 14-16, 2015 Orlando, USA

Jin-Ming Lin1, Chen Luo1, Yangdong Zhang1, Fengming Chen1,2, Katsumi Uchiyama2

1Tsinghua University, China 2Tokyo Metropolitan University, Japan

Posters-Accepted Abstracts: Organic Chem Curr Res

Abstract :

Applications of droplets for studies in small culture volume, especially microdroplets in microfluidics, have aroused the interests ofphysical, chemical and biological fields. During the last 10 years, various methods for forming and manipulating small droplets have been developed. Each droplet provides a compartment in which species or reactions can be isolated and therefore is suitable for quantitative studies. Furthermore, high-throughput experiments with extremely small volumes, single molecules, or single cells can also be achieved through droplet-based systems.Ambient ionization, which aims at direct sampling of analytes in the ambient state, has emerged rapidly in recent years. Among the latest progresses in atmospheric pressure Electrospray-based Ionization (ESI) techniques, paper-based ESI is highly promising in consideration of its simplified protocol of sample preparation and equipment. Capability of paper-basedESI-MS for the analysis of drugs, peptides, nucleotides and phospholipids in complex biological fluid samples, such as whole blood and raw urine, has been demonstrated recently. And the transport and ionization mechanisms, the appropriate substrate and solvent for effective paper-based ESI were investigated. Therefore, coupling microfluidics and mass spectrometry can combine the advantages of these two techniques, offering a new platform for scientific research. In this work, a paper, spray mass spectrometry combined with droplets generated by gravity and electrostatic attraction, microfluidic chip, and inkjet methods, was developed. The qualitative and quantitativeanalytical performances of this technique for single droplet were demonstrated. This manually controlled interface is straightforward, low-cost and simple to implement. Moreover, paper spray ionization mass spectrometry hold promises in direct analysis of real biological/chemical microreaction samples because of its tolerance with complex matrix. As a proof-of-concept example, the droplet-based acetylcholine hydrolysis werecarried out to demonstrate the validation of our method for direct analysis of micro-chemical/biological reactions..

Biography :

Email: jmlin@mail.tsinghua.edu.cn