Gynecology & Obstetrics
Open Access
ISSN: 2161-0932
ISSN: 2161-0932
Amal S El-Shal, Sally M Shalaby, Nader M Aly, Nearmeen M Rashad and Ahmed M Abdelaziz
Scientific Tracks Abstracts: Gynecol Obstet (Sunnyvale)
Obesity, insulin resistance, and hyperandrogenismare considered crucial parameters of polycystic ovarysyndrome (PCOS) which might be related to vitamin Dmetabolism. The aim of this study was to investigate theassociations between polymorphisms (TaqI and ApaI) inthe vitamin D receptor gene (VDR) and PCOS amongEgyptian women. We aimed also to elucidate the impact ofthese polymorphisms on vitamin D level, hormonal andmetabolic parameters of PCOS. One hundred and fiftyEgyptian women with PCOS and 150 unrelated controlswere enrolled in this study. Polymorphisms of VDR Taq- IT/C (rs731236) and Apa-I A/C (rs7975232) gene weregenotyped using polymerase chain reaction restrictionfragment length polymorphism (PCR-RFLP). Serum 25hydroxy vitamin D [25(OH) D] levels were measured byhigh-performance liquid chromatography. PCOS women. Had significantly lower levels of 25(OH) D compared tohealthy women. Our results revealed that Taq-I CC genotypeand C allele were associated with increased risk ofPCOS, while the Apa-I polymorphism was not. HaplotypeTaq-I C/ Apa-I C was associated with a higher PCOS riskmore than controls. Moreover, there was a significantdecrease of 25(OH) D levels in carriers of haplotype Taq-I C/ Apa-I C (with variant alleles) compared to the noncarriers.Results showed also that there was an obesity-VDR Taq-I genotypes interactions. These results suggestedthat, VDR Taq-I gene polymorphism is associated withincreased risk of PCOS in Egyptian women.