Journal of Cell Science & Therapy
Open Access

Glucose metabolism and vascular progenitors: A link to stem cell kinetics

2^nd World Congress on Cell Science & Stem Cell Research

November 12-14, 2012 Hilton San Antonio Airport, USA

Madhulika Dixit

Scientific Tracks Abstracts: J Cell Sci Ther

Abstract :

C D34+, CD133+ and CD34+CD133+ hematopoietic stem cells exhibit extreme plasticity. They give rise to progenitors of the cardiovascular system such as endothelial progenitor cells (EPCs), cardiomyocytes and smooth muscle cell progenitors. These cells follow circadian kinetics and get mobilized from bone marrow into circulation upon receipt of physiological cues such as tissue ischemia. Abnormalities in carbohydrate metabolism form a continuum which progressively worsens as a subject transitions from healthy to pre-diabetic and from pre-diabetic to diabetic state. Similarly, altered glucose metabolism is the underlying feature of reproductive endocrinopathy observed in polycystic ovary syndrome patients. Although these progenitors are negatively correlated with risk factors of metabolic syndrome, not much is known about their mobilization from bone marrow in disease states. We will present data on influence of oral glucose challenge on circulating numbers of CD34+, CD133+ and CD34+CD133+ cells in healthy versus insulin resistant subjects. In vitro culture of peripheral blood derived mononuclear cells in endothelial specific growth medium, EGM-2, is a surrogate marker for analyzing the functional ability of circulating endothelial progenitor cells with regard to their endothelial differentiation and angiogenic potential. We hypothesized that in subjects with pre-diabetes and polycystic ovaries the endothelial differential potential of circulating EPCs would be compromised. We observed that although the cultured cells had normal LDL uptake as assessed by dil-Ac-LDL labeling, they exhibited reduced nitric oxide levels. We will present data summarize these findings

Biography :

Madhulika Dixit received her PhD degree from Indian Institute of Technology Bombay in 2002. She has published numerous peer-reviewed papers in reputed journals and serves as reviewer for relevant journals. She is the recipient of the Senior IYBA award instituted by Ministry of Science and Technology, Government of India. Her current research interest includes elucidation of molecular and cellular mechanisms promoting dysfunction of vascular progenitor cells