Journal of Clinical Toxicology
Open Access
ISSN: 2161-0495
+44 1478 350008
ISSN: 2161-0495
+44 1478 350008
Michal H Wrobel
Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Poland
Scientific Tracks Abstracts: J Clin Toxicol
Glyphosate based herbicides are intensively used in modern agriculture. Therefore, the aim of the study was to investigate the
effect of the pure glyphosate and its most popular product (Roundup) on hormonal regulation of the force of myometrial
contractions. The myometrial, endometrial, granulosa and luteal cells, as well as the strips of myometrium, from non-pregnant
cows, were incubated with the herbicides at doses (0.1, 1 or 10 ng/ml) which were closed to their environmental amount.
Neither glyphosate nor Roundup affected the viability of studied cells. Glyphosate stimulated the secretion of testosterone
(T) and estradiol from granulosa cells, while Roundup inhibited the T secretion. Both compounds decreased the secretion
of progesterone while increased oxytocin (OT) secretion from luteal cells. Roundup significantly decreased prostaglandins
(PGF2 and PGE2) secretion from endometrial but not myometrial cells. However, neither basal nor OT-stimulated myometrial
contractions were affected by both compounds. The data show that the herbicides impaired the secretory function of ovarian
and endometrial cells. Admittedly both compounds change the amount of regulators of uterine contractions, but they do not
affect the force of myometrial contractions directly. The data clearly show that these herbicides can support the luteolytic
process but may only indicate the potential of these compounds to disturb the regulation of smooth muscle motility.
The work is supported by National Science Centre (Project N N311 082140), Polish Academy of Sciences (GW29) and by the
grant of KNOW Consortium "Healthy Animal � Safe Food", MS&HE Decision No. 05-1/KNOW2/2015
MichaÃ?Â? H Wróbel is currently an Associate Professor at the Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences in Olsztyn (Poland). He earned his PhD in Faculty of Biology from the University of Warmia and Masury in Olsztyn, in 2008. In his thesis work he pioneered the adverse effect of chlorinated xenobiotics (polichlorinated biphenyls and pesticides) on bovine myometrial contractions. After completing his MSc, he worked as an Assistant Professor in the Department of Cattle Physiology and Endocrinology of the Institute of Animal Reproduction and Food Research, PAS. In 2009, he became an Associate Professor in the Department of Physiology and Toxicology of Reproduction of the Institute of Animal Reproduction and Food Research, PAS.
Email: m.wrobel@pan.olsztyn.pl