Journal of Clinical and Cellular Immunology
Open Access

Head and neck squamous cancer stem cells and metastasis in vitro and in vivo in the chick chorioallantoic membrane assay

10^th Global Summit on Immunology and Cell biology

May 11-12, 2018 Osaka, Japan

Young-Hoon Joo and Thomas E Carey

The Catholic University of Korea, South Korea
University of Michigan, USA

Posters & Accepted Abstracts: J Clin Cell Immunol

Abstract :

Background: To evaluate the impact of CSC proportion on metastasis in Head and Neck Squamous Cell Carcinoma (HNSCC) using in vitro and in vivo models. Method: In vitro motility was assessed using wound healing and matrigel invasion assays. Tumorigenesis and metastasis were assessed in a Chick Chorioallantoic Membrane (CAM) assay. Results: Flow cytometry demonstrated 60-70% CD44+ cells in UMSCC-110 and 30-40% in UMSCC-106. UMSCC-110 cells demonstrated significantly faster wound closure (p<0.05) and more efficient invasion (p=0.033) compared to UMSCC-106 cells. In the CAM assay, there was a significant difference in liver metastasis between CD44high cells and unsorted or CD44low cells (p=0.027 in UMSCC-110 and p=0.035 in UMSCC-106, respectively). Conclusion: The proportion of CSC was significantly related to mobility and metastasis in HNSCC cell lines. This concept might help to improve understanding of mechanisms of metastasis and change therapeutic approaches to treating cancer metastasis. joodoct@catholic.ac.kr