Journal of Clinical Toxicology
Open Access

Heavy metal-induced up regulation of anti-oxidative enzymes and hemolymph biochemical compositions in Oxya hyla hyla (Serville)

4^th Global Summit on Toxicology

August 24-26, 2015 Philadelphia, USA

Iqra Azam, Sumera Afsheen and Muhammad Kaleem Sarwar

Posters-Accepted Abstracts: J Clin Toxicol

Abstract :

Present study was conducted to determine toxic effects of heavy metals (Cd, Pb and Hg) on antioxidant enzymes of a grasshopper
species i.e., Oxya hyla hyla (Serville). Changes in the activities of antioxidant enzymes [superoxide dismutase (SOD), catalase
(CAT), peroxidase (POD), lipid peroxidation (LPO)] and biochemical composition of hemolymph were measured by exposing trailed
insect to Cd+2, Pb+2 and Hg+2 at different concentrations (0, 0.50×10-4, 1.10×10-4, 1.55×10-4gg-1) of CdCl2, PbCl2 and HgCl2
for variable exposure time (24, 50 and 75 hours). The insect showed significant accumulation of metals with the increase inexposure
of dose and time. The SOD activity was lowered at 1.10×10-4 to 1.55×10-4gg-1 than at 0.50×10-4gg-1 Cd+2, Pb+2 and Hg+2 levels.
Detoxification effect was expressed by SOD at low metal concentrations and this effect disappeared at high concentrations. Assayed
activities of CAT, POD and LPO level were significantly accelerated and correlated positively as metal exposure time increased
showing species dependency. A significant decrease in total soluble protein, sugar, lipids and glycogen contents was observed due
to metal exposure throughout the entire tested period except after the first 24 hours of exposure at the lower concentrations. Thus
CAT had a strong detoxification effect as compared to SOD whereas POD had a weak detoxification effect. The study thus concludes
that O. hyla hyla (Orthoptera: Acrididdae) and its antioxidant enzyme level can be used as bioindicator and bio marker of biotic and
abiotic stresses.