Internal Medicine: Open Access
Open Access
ISSN: 2165-8048
+44 1300 500008
ISSN: 2165-8048
+44 1300 500008
Cityta Putri Kwarta
Airlangga University, Indonesia
Posters & Accepted Abstracts: Intern Med
Chronic Low Back Pain (CLBP) approximately 60-80% of the world population has ever experienced it at least once during their lifetime. Injectable hydrogels are the newest way to restore the disc thickness and hydration caused by disc degeneration by means of minimally invasive surgery. The needed polymers must also have biocompatibility, degradation properties and biological responses that are good for the body, so that the combination of thermo-responsive polymers with natural polymers can be a strong candidate of gelling. Thus, those types of polymers can be combined to improve the characteristic properties of injectable hydrogels, leading to the use of hyaluronic acid (a natural polymer) and polyethylene glycol (PEG) with the Horse Radish Peroxide cross-linker (HRP) enzymes. The swelling test results using the PBS, which approaches the ideal disc values were samples with variation of enzyme concentrations of 0.25 �¼mol/min/mL, which is 33.95%. The degradation test proved that the sample degradation increased along with the decrease of the HRP enzyme concentration. The results of the cytotoxicity assay with MTT assay method showed that all three samples resulted in 90% of living cells are not toxic. In vitro injection models demonstrated that higher the concentration of the enzymes, the less the state of the gel would rupture when released from the agarose gel. The functional group characterization shows cross-linking bonding in sample with enzyme adding. The conclusion of this study is the PEG-HA-HRP enzymes are safe polymer composites that have the potential to be applied as an injectable hydrogel for intervertebral disc degeneration.
Email: citytaputrikwarta@gmail.com