Virology & Mycology
Open Access
ISSN: 2161-0517
ISSN: 2161-0517
Ayatollah Nasrollahi Omran, Alinaghi Ghiasi and Negin Naghdi
Islamic Azad University, Iran
Posters & Accepted Abstracts: Virol Mycol
Introduction: Acne is a pathological disorder and a chronic inflammation in the sebaceous follicles and one of the most popular dermatology damages that has affected millions of people worldwide. Bacterial and fungal skin flora agents involved in its creation. Aim: In this study we aimed to identify and investigate drug-resistant yeast species isolated from the skin of patients with acne clinical protests referred to the dermatology clinic in west Mazandaran. Method: In this cross-sectional study of 70 clinical specimens from suspected skin with acne protests were collected by sterile swab and were streaked on SDA medium containing chloramphenicol. The plates were incubated for 48 hours in 37 oC. Suspected colonies were studied through microscopic examination and subsequent passage in accordance with mycology of standard procedures and specify the type of fungal colony color in CHROM agar for the isolation of the yeast. For final approval Candida species sequencing method was performed and susceptibility testing was performed to review Candida for drug-resistant isolates based on CLSI method. Result: Of 70 clinical isolates studied, 11 species of Candida including C. parapsilosis 8 (72.73%), C. krusei 1 (12.5%), C. lusitaniae 1 (12.5%), Candida kefir 1 (12.5%) and a Trichosporon asahii were identified and isolated. C. parapsilosis isolates susceptibility to various concentrations of the antifungal agents to isolate Cp1 study has shown that the isolated Cp8, Cp5 with MIC5 equal to 32, 0.5, 0.25 and MIC90 of <64, <1, <0.5 �¼g/ml fluconazole, itraconazole and ketoconazole were respectively resistant. Apart from the isolation of Cp1 and Cp8 which had relative strength almost all other species of C. parapsilosis isolates were susceptible to these drugs. Conclusion: Etiological factors, pathogenesis, drug resistance and risk factors of acne and the role of yeast to induce skin disease as a contributory factor in causing acne can be a topic of interest in dermatology.
Email: AYAT51@yahoo.co.in