Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
ISSN: 2155-9899
Michail Ponizovskiy
Accepted Abstracts: J Clin Cell Immunol
During angiogenesis, interaction of endothelial cells (EC) with cells of microenvironment, including monocytes/ macrophages, and the extracellular matrix are controlled by cytokines. The aim of the research was investigation of cytokine influence on capillary-like tube formation of endothelial cells EA.Hy926 in presence of THP-1 cells. For cell activation the following recombinant cytokines were used: bFGF - (BD, США) (1 ng/ml, 10 ng/ml, 20 ng/ml); VEGF - (BD, США) (1 ng/ ml, 10 ng/ml, 100 ng/ml); PlGF - (BD, США) (1 ng/ml, 5 ng/ml, 20 ng/ml); TGFβ - (BD, США) (1 ng/ml, 5 ng/ml, 10 ng/ml). Endothelial cells were seeded on Matrigel-coated 24-well plates (BD, USA) at a density of 150000 cells/well, also were added cytokines and 2,5% fetal bovine serum. To part of wells THP-1 cells were added (250000 cells/well), to part of wells - the cultural medium without THP-1 cells. Capillary tube formation was assessed 24 hours later using microscope AxioObserver Z1. Stimulating effect of bFGF and VEGF on capillary-like tube formation in monoculture and co-culture system was shown, while the effects of cytokines were more pronounced in co-culture system. PlGF had no effect on EC tube formation in monoculture, but at high concentrations proangiogenic activity of PlGF in co-culture system was shown. TGFβ inhibited EC tube formation, while its anti-angiogenic potential was more pronounced in the co-culture system. Thus, cytokines of microenvironment could make a definite contribution to the interaction of EC and monocytes/macrophages during angiogenesis.