Applied Microbiology: Open Access
Open Access

ISSN: 2471-9315

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In vivo formation of Candida biofilms on catheters surfaces: The nosocomial fungal infectivity


4th World Congress and Expo on Applied Microbiology

September 19-21, 2016 Las Vegas, USA

Sidi Mohammed Lahbib Seddiki, Z Boucherit-Otmani, R A Bettioui, K Boucherit, M Taleb and D Kunkel

University of Tlemcen, Algeria
University Center of Naama, Algeria
University Hospital of Sidi Bel Abbes, Algeria
Dennis Kunkel Microscopy Inc., USA

Posters & Accepted Abstracts: Appli Micro Open Access

Abstract :

The invasive nosocomial infections due to Candida species are responsible for increasing the length of stay, cost of hospitalization and morbidity in immunocompromised patients. Their severity and rapid progressivity are owed to the difficulty of diagnosis. Various catheters, which are often used to train a body fluid (blood, urine, infusion, parenteral nutrition, medication) inside the body of the patient or vice versa, are susceptible to be altered by Candida spp. and promote the formation of biofilms which consolidates the risk of invasive nosocomial infections i.e., these structures are considered as a nest for disease because it is not easily eradicate by conventional antifungal therapy. Such as the diagnosis of candidiasis related to catheter is difficult, the differentiation between catheter infection and a simple contamination is essential to establish an antifungal treatment. Our study aimed adapts to yeasts the Brun-Buisson (1987) method which only concerned by bacteria, thatâ��s why we conducted our study between February 2011 and January 2012 at the Hospital University Center of Sidi Bel Abb�¨s-Algeria that aims to evaluate the various types of fungal catheters infectivities (contaminations, colonization and infections) and their corresponding rates, as well as the responsible yeast species. At the end, the ability to form biofilms was checked. The results showed that three types of fungal infectivities of catheters were identified. On the other hand, SEM images showed clearly Candida biofilms on the surfaces of catheters.

Biography :

Email: seddiki.med@gmail.com

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