Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
ISSN: 2155-9899
Lvova T Yu, Stepanova O I, Okorokova L S, Beljakova K L, Selkov S A and Sokolov D I
Accepted Abstracts: J Clin Cell Immunol
Macrophages play an essential role in angiogenesis regulation. The aim of the research was investigation of THP-1 cells influence on capillary-like tube formation of endothelial cells EA.Hy926 (EC) at presence of soluble placental products obtained from healthy pregnant women on 9-11 weeks of gestation (n=20, group 1) and from healthy pregnant women on 38-39 weeks of gestation (n=20, group 2). EC were seeded on Matrigel-coated 24-well plates (BD, USA) at a density of 150000 cells/well, also were added placental tissue supernatants and 2,5% fetal bovine serum. To part of wells THP-1 cells were added (250000 cells/well), to part of wells - the cultural medium without THP-1 cells. Tube formation was assessed 24 hours later using microscope AxioObserver Z1. Addition of THP-1 cells to the EC cultivated in the presence of soluble placental products of group 1 resulted in increasing of tube length and reducing of tube number, while in the presence of soluble placental products of group 2 addition of THP-1 cells did not lead to changes in length and number of tubes. In monoculture system and in co-culture system were established increasing of tube length in the presence of soluble placental products of group 2 compared to group 1. Our data suggest that THP-1 cells can modulate tube formation of EC in the presence of soluble placental products. Obtained data may reflect the influence of monocytes/macrophages on EC tube formation in placenta at different stages of physiological pregnancy.