Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
ISSN: 2155-9899
Mehrdad Halaji, Jamshid Faghri, Samereh Nouri, Saba Jalalifar and Mehrdad Zalipoor
Isfahan University of Medical Sciences, Iran
Posters & Accepted Abstracts: J Clin Cell Immunol
Objectives: The role of integrons in the transfer of antibiotic resistance is one of the important issues, therefore, this study
is aimed to investigate antibiotic resistance pattern and prevalence of class 1 and 2 integrons in P.aeruginosa isolated of
nosocomial infection.
Material and Methods: P.aeruginosa isolates were collected during the April 2016 to August 2016 at a teaching hospital
affiliated to Isfahan University of Medical Sciences, Isfahan, Iran. Antibiotic susceptibility pattern was assessed using disk
diffusion method. PCR was carried out to detect the tox-A, class 1 and 2 integrons gene using the specific primers.
Results: A total of 72 confirmed P. aeruginosa isolates that half of them from ICU and most of the isolates were isolated
from trachea samples. Antibacterial susceptibility pattern showed that ceftazidime revealed the most resistance (76.4%)
and colistin was the most effective antibiotic (100%) and molecular analysis of class I and II integrons showed 55.5%
and 29.1% of isolates were positive, respectively and The proportions of MDR isolates were significantly higher among
integron-positive isolates with 43.3% compared to negative isolates with 22.9%.
Conclusion: Our results showed relevance among class 1 and 2 integrons and MDR P. aeruginosa isolates. According to
the importance of integrons in acquisition and dissemination of antibiotics resistance genes among these pathogens, so, the
performance of antibiotic surveillance programs is recommended for control the spreading of antibiotics resistance genes.
E-mail: m.hallaji@resident.mui.ac