Biochemistry & Pharmacology: Open Access
Open Access
ISSN: 2167-0501
+44-77-2385-9429
ISSN: 2167-0501
+44-77-2385-9429
Ali Shaharki, Marzieh Lotfian Sargazi and Ramin Saravani
University of Sistan and Baluchestan, Iran
Zahedan University of Medical Sciences, Iran
Scientific Tracks Abstracts: Biochem Pharmacol (Los Angel)
Increased expression of cyclic guanosine 3', 5' monophosphate phosphodiesterase-5 (cGMP-PDEs) mRNAs has been
demonstrated in several human carcinomas, including breast carcinoma, pancreatic cancer, bladder cancer, squamous
cell carcinoma and prostate cancer, in comparison to adjacent normal tissues. Studies have elucidated that an increase in
intracellular cGMP induces apoptosis and decreases cell population growth. Therefore, selective inhibitors of these PDEs
might be potential anticancer agents. Previous investigations have demonstrated that Levisticum officinale contains flavonoids
and alkaloids compound that are known as antitumoral components. The purpose of this study was to investigate the effect of
Levisticum officinale hydroalcoholic extract on phosphodiesterase 5 gene expression, cGMP signaling pathway and its role in
inducing apoptosis in the MCF-7(ER+) and in MDA-Mb-468 (triple-negative) cell lines. The mean inhibitory concentration
(IC50) of extract was determined in both cell lines using of MTT assay, and the type of cell death was detected by flow
cytometry. The expression of PDE5 and cGMP levels was measured by real-time polymerase chain reaction and colorimetric
assay, respectively. Treatment with hydroalchoholic extract of Levisticum officinale showed that 200 �¼g/ml to be the IC50 for
both cell lines. 12 hour treatment with IC50 dosage showed a maximum decrease in the PDE5 expression and maximum
increase in intracellular cGMP level, although, these effects were more significant in MDA-MB-468. In conclusion, our results
showed that hydroalchoholic extract of Levisticum officinale had an anti-proliferative and apoptotic effect in MCF-7 and MDAMb-
468 targeting PDE5 and cGMP signaling pathway.
Recent Publications
1. Saravani R Galavi H R and Shahraki A (2017) Inhibition of phosphodiesterase 5 and increasing the level of cyclic guanosine 3',
5' monophosphate by hydroalchoholic Achillea wilhelmsii C. Koch extract in human breast cancer cell lines MCF-7 and MDAMb-
468. Breast Cancer: Basic and Clinical Res. 1�7.
2. Afarnegan H, Shahraki A and Shahraki J (2017) The hepatoprotective effects of aquatic extract of L. officinale against paraquat
hepatocyte toxicity. Pak J Pharm Sci 30:2363�2368.
3. Lagzian M, Shahraki A, Besharatian M and Asoodeh A (2017) A thermostable alkaliphilic protein-disulfide isomerae from
bacillus subtilis DR8806: cloning, expression, biochemical characterization and molecular dynamics simulation. Int J Biol
Macromol. 09:033.
4. Sargazi S, Saravani R, Galavi H R, et al. (2017) Effect of Levisticum officinale hydroalcoholic extract on DU-145 and PC-3
prostate cancer cell lines. Gene Cell Tissue 4(4):e66094.
5. Galavi H R, Saravani R, Shahraki A and Ashtiani M (2016) Anti-proliferative and apoptosis inducing potential of hydroalchoholic
Achillea wilhelmsii C. Koch extract on human breast adenocarcinoma cell lines MCF-7 and MDA-Mb-468. Pak J Pharm Sci 29:
2397�2403.
Ali Shahraki has his expertise in Teaching and Research at the University of Sistan and Baluchestan, Zahedan, Iran. He supervised more than 25 theses of Biochemistry for Master’s Degree of Science students. He works in the fields of neuroscience, herbal medicine and signal pathways for inducing anti-proliferative and apoptosis in cancer cell lines through medicinal plants. He has built this model after years of experience in research, evaluation, teaching and administration in universities and education institutions.
E-mail: ashahraki@science.usb.ac.ir