Applied Microbiology: Open Access
Open Access
ISSN: 2471-9315
+44 1300 500008
ISSN: 2471-9315
+44 1300 500008
Hala Ibrahim Mersa Al-Daghistani, Walid D Shquirat and Muna Al-Kharabsha
Al-Balqa Applied University, Jordan
Posters & Accepted Abstracts: Appli Micro Open Access
Staphylococcus aureus is the major bacterial cause of skin, soft tissue and bone infections and one of the commonest causes of healthcare-associated bacteremia. Vancomycin use has used worldwide as a result of empirical therapy against methicillinresistant S. aureus (MRSA) infections. This will create a selective pressure that favors the outgrowth of vancomycin-resistant (VRSA) and vancomycin intermediate S. aureus clones (VISA). This study was carried out to evaluate the resistance pattern of S. aureus to vancomycin. A total of 1179 samples including 566 (48%) from human and 613 (51.90%) from animals were examined for the presence of S. aureus using standard biochemical tests and PCR amplification of coa gene. Their prevalence among human source was: 19.35%, 14% and 8.8% for nasal, nail and skin, respectively. However, animal sources showed: 27.3%, 5.51% and 15.86% for milk, nasal and meat, respectively. Resistance to antibiotics was determined by the disk diffusion method. MRSA strains were tested for vancomycin resistance by minimal inhibitory concentration (MIC) and E-test. Four human isolates (1.87%) have shown to be VISA. The isolates were belongs to the nose (4.76%), nails (3.2%) and clinical samples (1.56%). VISA strains were also resistance to erythromycin, methicillin, TMP and oxacillin. The results were confirmed by PCR amplification of vanA, vanB, vanC genes. VanB gene was found in all VISA strains. They all exhibited the same PFGE pattern. Though incidence was not so high but this is believed to be the first report of VISA strains containing vanB isolated from a routine nasal and nail carriage.
Email: hala2002dagh@yahoo.com