Virology & Mycology
Open Access

Lactobacillus stimulates intestinal epithelial proliferation to repair mucosal barrier through activation of Wnt/β-catenin pathway

Joint Event 10^th International Virology Summit & 4^th International Conference on Influenza & Zoonotic Diseases

July 02-04, 2018 | Vienna, Austria

Lulu Ye, Qinghua Yu and Weigang

Nanjing Agricultural University, China

Posters & Accepted Abstracts: Virol Mycol

Abstract :

The detailed protective mechanisms of Lactobacillus on intestinal mucosa, especially from proliferation of intestinal stem cells, are still incompletely understood. In this study, intestinal organoids and mice were used to explore protective effect of Lactobacillus on intestinal mucosal barrier. We found that L. acidophilus increased survival ratio of mice infected with S. typhimurium, as well as reduced IL-1�² and TNF-�± secretion and S. typhimurium colonization in feces. S. typhimurium infection caused crypt hyperplasia with enhancement of PCNA+ cells and crypt length remarkably with over-expression of �²-catenin, which was also remitted by L. acidophilus administration. Similar protective effects of L. acidophilus on inhibiting intestinal inflammation and crypt hyperplasia induced by S. typhimurium infection was also observed in intestinal organoids by alleviating TNF-�± secretion and �²-catenin over-expression. S. typhimurium infection caused UEA-1+ goblet cells and Lysozyme+ Paneth cells hyperplasia both in mice and organoids to defend against S. typhimurium invasion through exploiting TLR2 and TLR4. However, L. acidophilus ATCC 4356 ameliorated over-activation of TLRs and Wnt/�²-catenin pathway to impair excessive expansion of goblet cells and Paneth cells induced by S. typhimurium. Our study implied that S. typhimuriumprotected intestinal mucosa against S. typhimurium infection by modulating Wnt/�²-catenin pathways to affect intestinal epithelial proliferation..

Biography :

 

E-mail: yuqinghua1981@njau.edu.cn