Drug Designing: Open Access
Open Access

Low-dose of bisphenol: A disrupts steroidogenesis through transcriptional regulation in adrenal and placenta

9^th Annual Congress on Drug Design & Drug Formulation

October 19-20, 2017 Seoul, South Korea

Hsin-Chieh Lan

National Defense Medical Center, Taiwan

Posters & Accepted Abstracts: Drug Des

Abstract :

Bisphenol A (BPA) is an industrial material used for many plastic products and is considered as an endocrine disruptor. BPA can be released into the environment and can spread through the food chain. BPA levels in human serum are commonly around 1-100 nM. It is well known that BPA exposure leads to lesions, especially in the endocrine and reproductive systems. According to previous studies, BPA disrupts ovary and testis function and induces cell apoptosis. However, BPA effects in adrenal and placenta are less understood. Placenta secretes important hormones, such as progesterone and estrogen, to maintain gestation. Adrenal is the most important organ that responses stress by producing glucocorticoid. Both adrenal and placenta express specific enzymes for steroid hormone synthesis, such as P450scc (CYP11A1) which converts cholesterol to pregnenolone and aromatase (CYP19) which induces androgen conversion to estrogen. To determine the effects of a low dose of BPA on hormone synthesis in placenta and adrenal, gene expressions of CYP11A1 and CYP19 were measured in cell culture system. The hormone levels secreted in cultured medium were detected by ELISA as the final indicator of steroidogenesis. Our data demonstrated that treatment with a low dose of BPA does not affect cell survival, but the hormone production, CYP gene activation and signaling pathways are changed.