Journal of Drug Metabolism & Toxicology
Open Access

mecA sequencing and SCCmec typing in methicillin resistant Staphylococci: A highly diversified element with new mutations in mecA

3^rd World Congress and Exhibition on Antibiotics and Antibiotic Resistance

July 31-August 01, 2017 | Milan, Italy

Ferhat Djoudi, Celestino Bonura and Said Benallaoua

University of Bejaia, Algeria
University of Palermo, Italy

Posters & Accepted Abstracts: J Drug Metab Toxicol

Abstract :

Genetic mechanisms of methicillin resistance are still relevant in Staphylococci. The aim of this study is to assess the possible exchanges of staphylococcal cassette chromosome mec (SCCmec) between isolates of Methicillin-Resistant Staphylococci (MRS) and to check for known or new mutations in mecA DNA. A total of 35 MRS non repetitive isolates were recovered, including 20 S. haemolyticus, 7 S. aureus, 4 S. sciuri, 2 S. saprophyticus, and one isolate each of S. xylosus and S. lentus. Only 16 of the 35 strains were assigned to known SCCmec types: 7 SCCmec VII, 6 SCCmec IV and 3 SCCmec III, with possible horizontal transfer of the SCCmec VII from methicillin-resistant S. haemolyticus to methicillin-susceptible S. aureus. mecA genes sequencing in 10 selected isolates allowed description of nine punctual mutations, seven reported for the first time. The most frequent mutation was G246E, identified in isolates of methicillin-resistant S. aureus, S. sciuri, S. saprophyticus and S. lentus. These results emphasized the high degree of genetic diversity of SCCmec element in methicillin-resistant Staphylococci and describe new missense mutations in mecA, which might be important in understanding the evolution of methicillin and new �²-lactams resistance.

Biography :

Email: djoudi.ferhat@gmail.com