Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
ISSN: 2155-9899
Ponizovskiy M R
Accepted Abstracts: J Clin Cell Immunol
Natural Killer (NK) cells are dominant effectors of early host defense. Their activity depends on balance of signals from activation and inhibition receptors. Various soluble factors, including cytokines have an influence on phenotype of NK cells.To investigate this effect, we used pro-inflammatory and anti-inflammatory cytokines. IFN-γ induces Class II major histocompatibility complex (MHC) molecule expression. IL-8 basic functions include chemotaxis, activation and the induction of neutrophil infiltration. IL-10 is an anti-inflammatory cytokine with multidirectional effects in immunoregulation and inflammation. Assay was performed using NK-92MI natural killer cell line. Cells were treated with 3 different doses of cytokines (IFN-γ, IL-8, IL-10, separately) for 24 hours. Surface molecules expression was assessed by flow cytometric analysis using fluorochrome-conjugated mABs and FACSC. IFN-γ significantly (p<0.05) upregulated expressions of KIR2DL1, KIR3DL1, CD11a, CD11b, CD18, CD29, CD44 (dose-dependent), CD47 (dose-dependent), CD184 (dose-dependent), CD49d and CD54. Cells stimulated byIL-8 showed significally higher expression level ofCD29. However, IL-8 downregulated KIR2DL3, KIR2DL4, KIR3DL1 (dose-dependent), KIR2DS4, CD11b (dose-dependent), CD18, CD49d (dose-dependent), CD44 and CD58. Significantly increased expression of CD47, CD58 (dose-dependent) and CD49 was discovered in cells treated with IL- 10, but expression of KIR2DL1, KIR2DL3, CD29, CD44, and CD184 was significally decreased.
Larisa Viazmina is a five-year student of biotechnology faculty of Saint Petersburg Chemical Pharmaceutical Academy. She is laboratory research assistant in immunology department of D. O. Ott Research Institute of Obstetrics and Gynecology. Her research interests are related to reproductive immunology, mainly preeclampsia and NK cells.