Journal of Clinical and Experimental Ophthalmology
Open Access
ISSN: 2155-9570
ISSN: 2155-9570
Yu Qin, Jinsong Zhang, Jiangyue Zhao, Jing Li and Xinwei Wu
Posters-Accepted Abstracts: J Clin Exp Ophthalmol
Objective: To explore the expression of microRNA in Eaf2 knockout mouse and the effect of Eaf2 on both human lens epithelial cell
apoptosis and the expression of microRNA in lens.
Methods: pEGFP-C1-Eaf2 was transfected into SRA01/04 cells using Lipofectamine 2000 to over express the expression of Eaf2
gene, and then flow cytometry was used to detect cell apoptosis rate. While real time q-PCR was used to measure the expression of
microRNA both in human lens epithelial cell and Eaf2 knockout mouse.
Results: Compared with controls, the apoptosis rate of cells transfected with pEGFP-C1-Eaf2 was reduced. The expression of miR-
125b and let-7a was significantly increased, whereas miR-204 was decreased in cells transfected with pEGFP-C1-Eaf2. The expression
of miR-125b and let-7a was lower, while miR-204 was higher in Eaf2 knockout mouse. Each result was statistically significant (P<0.01).
Conclusion: Eaf2 might inhibit human lens epithelial cell apoptosis via regulating the expression of microRNA. Eaf2 may have a
protective effect of the lens in the genesis of cataract.