Biochemistry & Pharmacology: Open Access
Open Access

Method development and validation of ceftibuten in bulk and its pharmaceutical dosage form by RP-HPLC method

International Conference on Pharmacognosy, Phytochemistry & Natural Products

October 21-23, 2013 Radisson Blu Plaza Hotel, Hyderabad, India

Varaprasad Adepu and K. E. V. Nagoji

Accepted Abstracts: Biochem & Pharmacol

Abstract :

To establish a method and validation for the determination of ceftibuten in its pure form as well as dosage form by reverse phase high performance liquid chromatographic method. Chromatography was carried out on a Symmetry C18 (4.6x150 mm, 5μm, Make: XTerra) column using a mixture of phosphate buffer and acetonitrile HPLC grade (30:70) as the mobile phase at a flowrate 1.0 mL/min, the detection was carried out at 228 nm. The retention time of the drug was 2.430 minutes. The method produced linear response in the concentration range of 20 mcg/ml and 60 mcg/ml. The method precision for the determination of assay was below 1.0% RSD. The developed method was validated and this method is useful in the quality control of bulk and pharmaceutical formulations.