Journal of Food: Microbiology, Safety & Hygiene
Open Access
ISSN: 2476-2059
+44 1478 350008
ISSN: 2476-2059
+44 1478 350008
Zubair Ahmed
Mehran University of Engineering and Technology, Pakistan
Posters & Accepted Abstracts: J Food Microbiol Saf Hyg
The sugar industry is the largest industry in Pakistan, contributing 3.2% of GDP in agriculture. It produces alcohol and byproducts like molasses. However, it is also producing a significant amount of untreated wastewater, which is disposed of directly into canals by the distilleries and sugar manufacturer industries which causes serious problem to water resources and pollutes the environment. Only a few sugar industries are treating wastewater, but they have unsuitable treatment methods. Among wastewater and effluent treatment methods anaerobic digestion with a membrane bioreactor (AnMBR) is a most suitable method of treatment, in which waste is biologically broken down into biogas in the absence of oxygen, while filtration membrane helps to separate a tiny particle from wastewater sludge. In the present study, a pilot-scale AnMBR assembly was inoculated with sludge, taken from an anaerobic digester. The reactor was first accumulated using synthetic wastewater, followed by distillery wastewater. The pH of the reactor was maintained between 7.2 to 8.5. Two samples from the initial stage (AnMBR) of synthetic wastewater and two samples after the stabilizing the membrane bioreactor of distillery wastewater were collected. DNA extraction was carried out by using GeneJet DNA purification kit. For 16S rDNA-based metagenomics for bacterial community characterization, a V3-V4 region of 16S rRNA gene was amplified and amplicon libraries were sequenced by using Illumina MiSeq sequencer (Illumina Inc. USA). Quantitative insights into microbial ecology 2 (Qiime2) was used for quality control analyses, quality filtration, taxonomic characterization, alpha, and beta diversity analysis. Furthermore, fluorescence in situ hybridization (FISH) technique, comprising of eight bacterial oligonucleotide probes (labeled at 5â?? site with different fluorescent dyes, purchase from biomer.net) will also be used to detect microbial cells of different lineages. This study provides detailed insights into bacterial genera present in the sludge sample, responsible for favorable wastewater treatment.
E-mail: zahmed.uspcasw@faculty.muet.edu.pk