Journal of Cell Science & Therapy
Open Access
ISSN: 2157-7013
+44 1300 500008
ISSN: 2157-7013
+44 1300 500008
Haimin Li and Xisheng Yang
The Fourth Military Medical University, China
Posters & Accepted Abstracts: J Cell Sci Ther
Recently, microRNAs (miRNAs) have been linked to cancer metastasis. Although microRNA-200a (miR-200a) is frequently downregulated in human cancer, the role of miR-200a in side population (SP), cancer stem cells (CSCs) has not been investigated. In this study, 101 pairs of primary hepatocellular carcinoma (HCC) tissues and matched normal control tissues were analyzed for miR-200a expression and its clinicopathological value was determined. The SP cells of HCC cell lines were subjected to an in vitro/ vivo metastatic analysis. The expression of possible downstream targets of miR-200a in SP cells including that of the zinc finger E-box-binding homeobox (ZEB) protein was validated. Here, we found that miR-200a was down-regulated in HCC/SP and was related to metastasis. MiR-200a suppressed the metastasis of SP cells in vitro/vivo. The overexpression of miR-200a in SP cells reduced the expression of metastasis related markers and reduced the expression of ZEB2. The associations between miR-200a, SP cells and ZEB2 were validated in HCC. Collectively, these findings reveal that miR-200a may exert effects on the metastasis of SP cells via the transactivation of oncogene ZEB2.
Email: lihaim@fmmu.edu.cn