Journal of Antivirals & Antiretrovirals
Open Access
ISSN: 1948-5964
+44 1300 500008
ISSN: 1948-5964
+44 1300 500008
Manmohan Parida, Jyoti Shukla, Shashi Sharma and P.V.L. Rao
Scientific Tracks Abstracts: JAA
The development of accurate and rapid diagnosis is critical for minimizing further spread through timely implementation of control measures. A one -step single tube accelerated and quantitative S-OIV specifi c H1 RTLAMP assay is developed for clinical diagnosis of S-OIV by targeting the HA gene. Th e S-OIV RTLAMP was 10 fold more sensitive than WHO realtime RTPCR with a detection limit of 0.1 TCID 50/ml. Th e comparative evaluation of RTLAMP assay vis-a-vis WHO real-time RTPCR was undertaken with 1000 acute phase throat swab samples. Th e SOIV RTLAMP demonstrated exceptionally higher sensitivity by picking up more additional positive cases amongst the negatives that were further sequence confi rmed. Th e overall sensitivity and specifi city of the S-OIV specifi c H1 RTLAMP was 93% and 100% respectively. Further genetic diversity of the pandemic H1N1 viruses was studied through 188 bp partial HA gene sequence phylogeny. Th e phylogenetic analysis of pandemic H1N1 viruses of Gwalior region revealed 99.7% similarities with California and other circulating novel swine fl u viruses. 3 Major changes were observed at nucleotide level, while two major amino acid shift s were observed at the position C9W and I30M corresponding to the ORF with prototype strain. Th e phylogenetic analysis revealed the circulation of two genetically distinct lineages belonging to clade VII and I of S-OIV in central India during the 2009 and 2010 epidemics. Th us genetic diversity of these H1N1 viruses warrants continuous monitoring of the genetic makeup of this newly emergent virus to understand its evolution within the country.
Dr Manmohan Parida is presently working as a senior scientist in Defence Research and Development Establishment in the fi eld of advanced molecular diagnostics and epidemiology of circulating viruses for last 15 years. He has completed his Ph D from Jiwaji University, Gwalior in Microbiology in 2002. He has also worked as Monbusho Fellow and post doctoral researcher at Institute of Tropical Medicine, WHO reference centre for research on arboviruses under Nagasaki University, Japan. He has published more than 55 research papers in national and international journal of high repute. He has seven Indian and one Japanese patent to his credit.