Journal of Thyroid Disorders & Therapy
Open Access
ISSN: 2167-7948
+44 1300 500008
ISSN: 2167-7948
+44 1300 500008
Agnese Filippello
University of Catania, Italy
Scientific Tracks Abstracts: Thyroid Disorders Ther
Intestinal cell dysfunctions involved in obesity could be correlated with impaired intestinal cell development. To date, the molecular mechanisms underlying these dysfunctions have been poorly investigated because of the lack of a good model for studying obesity. The main aim of this study was to investigate the effects of lipotoxicity on intestinal cell differentiation in mouse intestinal organoids. Organoids were grown in the presence or absence of high palmitate to simulate lipotoxicity. Palmitate treatment altered the expression of markers involved in the differentiation of enterocytes and goblet cells in the early (Hes1) and late (Muc2) phases of their development, respectively, and it modified enterocytes and goblet cell numbers. Furthermore, the expression of enteroendocrine cell progenitors (Ngn3) and I cell (CCK) markers was also impaired, as well as CCK-positive cell numbers and CCK secretion. Our data indicate, for the first time, that lipotoxicity simultaneously influences the differentiation of specific intestinal cell types in the gut. Through this study, we identified novel targets associated with molecular mechanisms affected by lipotoxicity that could be important for obesity therapy.
Agnese Filippello PhD, has a major scientific interest in translational medicine and organoid research. She is an expert in organoid isolation from human and mouse tissues, molecular and cellular biology of organoids, and others cell lines. The main goals and responsibilities are represented by the molecular characterization organoids and 3D organoid live imaging involved in different types of diseases.