Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
ISSN: 2155-9899
Dina Antunes
Oswaldo Cruz Foundation, Brazil
Posters & Accepted Abstracts: J Clin Cell Immunol
Statament of the Problem: Oral transmission of Trypanosoma cruzi, the etiologic agent of Chagas disease, is the most important route of infection in Brazilian Amazon. Other South American countries have also reported outbreaks associated with food consumption. This route of transmission presents specific symptoms such as facial and lower limbs edema and, in some cases, bleeding and thrombosis. Nevertheless, studies that address this transmission are largely lacking regarding its pathogenesis and, more specifically, the crosstalk between immune and hemostatic systems. Methodology: BALB/c mice, aged 6�±8 weeks, were infected via oral cavity (Oral infection) with 5x104 metacyclic trypomastigotes Tulahu�©n strain, obtained from excreta of Triatoma infestans. Animals were handled according to the rules of the Ethics Committee for Animal Research of Oswaldo Cruz Foundation. Findings: When compared with control uninfected animals, orally infected mice presented high proinflammatory cytokine (TNF-�±, IFN-�³ and IL-6) serum levels. The highest concentrations were obtained concomitantly to the peak of parasitemia. Blood counts revealed thrombocytopenia in the oral infected group at 14 and 21 days post-infection (dpi) and leukocytosis at 21 dpi. Tail bleeding assay displayed that animals bled more at 21 dpi. Coagulation factor concentrations varied during infection with an increase in Factor V and Factor VIII consumption at 14 dpi indicating disturbances in the coagulation cascade. Activated partial thromboplastin time (aPTT) and Prothrombin time (PT) tests corroborated the hematological changes with an increase in clotting time at 21 dpi. Moreover, preliminary data indicate amelioration of the orally infected animals after blockage of IL-6 receptor, revealing a critical role of IL-6 in the course of oral infection. Conclusion & Significance: These results demonstrate, for the first time, that T. cruzi oral infection results in significant alterations in hemostatic system and indicates the relevance of the crosstalk between inflammation and hemostasis in this parasitic disease. Financial Support: Fiocruz, CNPq, Capes, Faperj (Brazil).
Dina Antunes has completed Master in Biotechnology at the Instituto Superior Técnico, Lisbon. She is currently a PhD in Cellular and Molecular Biology program, Immunopharmacology Specialization, at the Oswaldo Cruz Institute, Fiocruz in the Laboratory on Thymus Research (CNPq Scholarship). She has experience in Biotechnology, Biochemistry, Cellular and Molecular Biology and Immunology with emphasis on Chagas Disease and oral infection. In her PhD, she wants to find out if, in an experimental model of oral acute infection by T. cruzi, there are hematological changes that could explain bleeding disorders in patients with oral Chagas Disease and she studies the crosstalk with acute inflammation.
E-mail: dinadejesusantunes@gmail.com