Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
+44 1300 500008
ISSN: 2157-7064
+44 1300 500008
Biljana Skrbic, Jelena Zivancev
University of Novi Sad, Serbia
Posters & Accepted Abstracts: J Chromatogr Sep Tech
As acrude extract method followed by ultra-high performance liquid chromatography�tandem mass spectrometry (UHPLC� MS/MS) is a technique that has been frequently used as a routine analytical technique for mycotoxins in wheayt and corn, which applicability has been also demonstrated successfully on other matrices by the team, the intention of this presentation was to further provide the evidence on the method applicability for mycotoxin analysis of rice samples, for UHPLC�MS/ MS represents the most efficient and reliable analytical technique for high efficiency isolation, unequivocal identification and accurate quantification. Thus, in present investigation, a reliable UHPLC�MS/MS method was developed and validated for simultaneous determination of twelve mycotoxins (aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2, ochratoxin A, zearalenone, T-2 toxin, HT-2 toxin deoxynivalenol, citrinin, fumonisin B1 and fumonisin B2) in rice. One-step extraction using solvent mixtures of acetonitrile/water/acetic acid (79:20:1, v/v/v) without anyclean-up (i.e. crude extract method) was employed for themycotoxinsextraction from rice samples. Using matrix-matched calibration it has been demonstrated that the limits of quantification were below the relevantmaximum contents established by theEU regulation in rice or other cereals. The recoveries of the developed method were satisfactory between 96%�126% with relative standard deviation lower than 12%. The validated method was applied on real samples. The obtained results are used to estimate the exposure of theSerbian population to mycotoxins. To the best of our knowledge, this is the first report onoccurrence of the studied mycotoxins in ricecommercially availablein Serbia.
Email: biljana@tf.uns.ac.rs