Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
+44 1300 500008
ISSN: 2157-7064
+44 1300 500008
L A Kartsova, E A Bessonova, V F Gallyamova, A V Nikolaev and E V Obedkova
Saint-Petersburg State University, Russia
Posters & Accepted Abstracts: J Chromatogr Sep Tech
Our report is focused on the development of new analytical approaches for electrophoretic determination of biologically active substances with traditional CE and developed microfluidic chip-analyzer with different variants of on-line preconcentration. The use of hypercrosslinked polystyrene as a sorption material in the preparation of urine and blood serum for analysis provided the decrease in detection limits for hydrophobic and hydrophilic analytes. Application of watersoluble oligosaccharide derivatives hyperbranched polyethylenimine (PEI) as a covalent coating of silica fused capillary and combination of focusing principles of different variants on-line pre-concentration for analysis of proteins resulted in a 1100-fold improvement in sensitivity. The potential of long chain ionic liquids for on-line sample concentration techniques of ionogenic and neutral analytes in biological objects by different modes of capillary electrophoresis: zone (CZE) and micellar (MEKC) modes with normal and reversed polarity were investigated. The compounds chosen were biogenic amines and steroid hormones. Imidazolium-based ionic liquids C12MImCl, C16MImCl were used both as modifiers of electrophoretic systems and as pseudostationary phase. Sweeping with C16MImCl micelles in BGS has provided 83-112-fold sensitivity enhancement factors for catecholamines. It was found out that using highly conductivity sample matrix in sweeping leads to a significant increase in efficiency of analytes up to 1ΓΆΒ?Β?106 t.p. Chemometric processing of the obtained characteristic profiles of biologically active analytes of blood serum and urine samples from healthy donors and patients with endocrine diseases proved to be informative as an additional diagnostic criteria.
Email: bessonova.elena.a@gmail.com