Journal of Clinical and Experimental Ophthalmology
Open Access
ISSN: 2155-9570
ISSN: 2155-9570
Guzel Bikbova, Toshiyuki Oshitari and Shuichi Yamamoto
Scientific Tracks Abstracts: J Clin Exp Ophthalmol
Purpose: To determine the effect of advanced glycation end-products (AGEs) on neurite regeneration in isolated rat retinas and the
regenerative affects of different neurotrophic factors. Furthermore, to examine whether nuclear factor-�¸B (NF-�¸B) and specificity
protein 1 (SP1) expression are correlated with the regenerative effect of each neurotrophic factors.
Methods: Retinal explants of 4 adult SD rats were three-dimensionally cultured in collagen gel and incubated in: 1. Serum free
control culture media; 2. 100 μg/ml glucose-AGE-BSA, glycolaldehyde-AGE-BSA, glyceraldehyde-AGE-BSA media; 3. Glucose,
glycol, glycer+100 ng/ml neurotrophin 4 (NT-4) media; 4. Glucose, glycol, glycer+100 ng/ml hepatocyte growth factor (HGF) media.
5. Glucose, glycol, glycer+100 ng/ml glial cell lines derived neurotrophic factor (GDNF) media and 6. Glucose, glycol and glycer+100
ng/ml tauroursodeoxycholic acid (TUDCA) media; after 7 days, the number of regenerating neurites was counted under a phasecontrast
microscope. The explants were immunostained for NF-κB and SP1 transcription factors. Statistical analyses were performed
by one-way ANOVA.
Results: In retinas incubated with AGEs, the numbers of regenerating neuritis were fewer than in control. Neurotrophic factors
increased the number of neurites but more significantly in the NT-4 group. The numbers of NF-κB and SP1 immunopositive cells
were higher in retinas exposed to AGEs than in control. Neurotrophic factors decreased the number of NF-κB immunopositive cells
but did not significantly affect SP-1 expression. NT-4 shows the best neuroprotective and regenerative effect.
Conclusions: High dose AGEs impede neurite regeneration. The inhibition of regeneration is correlated with increased expression
of NF-κB and SP1. NT-4 enhances neurite regeneration in AGEs exposed retinas more than other neurotrophic factors such as HGF,
GDNF and TUDCA. The regenerative effect of NT-4 is correlated with NF-κB suppression.
Guzel Bikbova has completed her PhD from Scientific Research Institute of Eye Diseases Russian Academy of Medical Science, Moscow, Russia. She worked in Ufa Eye
Research Institute and now is obtaining another PhD in Chiba University, Japan as scholarship holder from Ministry of Education, Culture, Sport and Technology in Japan.
She has published 12 papers in peer reviewed journals and has 4 patents of Russian Federation.