Journal of Thyroid Disorders & Therapy
Open Access
ISSN: 2167-7948
+44 1300 500008
ISSN: 2167-7948
+44 1300 500008
Alessandra Scamporrino
University of Catania, Italy
Scientific Tracks Abstracts: Thyroid Disorders Ther
Type 2 diabetes and renal damage are strictly linked. The progressive increase in T2D incidence has stimulated the interest in novel biomarkers to improve the diagnostic performance of commonly utilized markers such as albuminuria and eGFR. Through the microarray method, we analyzed the entire transcriptome expressed in 12 serum samples of diabetic patients, six without ND and six with ND; the downregulation of the most dysregulated transcripts was validated in a wider cohort of 69 patients by qPCRs. We identified a total of 33 downregulated transcripts. The downregulation of four mitochondrial messenger RNAs (MT-ATP6, MT-ATP8, MT-COX3, MTND1) and other two transcripts (seysnoy, skerdo) was validated in patients with eGFR stage G3 versus G2 and G1. The four messenger RNAs correlated with creatinine and eGFR stages, while seysnoy and skerdo were associated with white blood cell values. All transcripts correlated also with Blood Urea Nitrogen. The four mitochondrial messenger RNAs had a high diagnostic performance in G3 versus G2 discrimination, with AUC values above 0.8. The most performant transcript was MT-ATP6, with an AUC of 0.846; sensitivity = 90%, specificity = 76%, p-value = 7.8 x 10-5. This study led to the identification of a specific molecular signature of ND, proposing the dosage of RNAs, especially mitochondrial RNAs, as non-invasive biomarkers of diabetes complications.
Alessandra Scamporrino studied genetics and molecular biology in basic and biomedical research at the University La Sapienza, Roma, Italy and graduated in 2014. She then joined the research group of Francesco Purrello at the Department of Clinical and Experimental Medicine. She received her phD. degree in complex systems in the physical, socio-economic and life sciences in 2020 at the University of Catania. As a postdoctoral fellowship (from 2020 to date) she has been working on several projects mainly focused on the identification of non-coding RNAs as circulating noninvasive biomarkers or intracellular novel molecular targets of several kinds of diseases.