Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
ISSN: 2155-9899
Puja Kumari and Himanshu Kumar
Indian Institute of Science Education and Research Sciences, Bhopal, India
Posters & Accepted Abstracts: J Clin Cell Immunol
Introduction: Human cytomegalovirus (HCMV) is found to be associated with many cancer types, since last two decades. However, a role of HCMV in cancer remains unknown. Here, we have investigated the role of HCMV in oncogenesis via inhibiting innate-immunity. Method: Deubiquitinase (DUB) sufficient- and deficient-HCMV were used to infect mammalian cell-line to analyze induction of oncogenic properties and inhibition of type I IFNs synthesis. Results were further confirmed by overexpression of WT-and Mutant-DUB domain of HCMV-DUB gene in mammalian cell-lines. Result: HCMV-DUB enhanced cellular metabolic activity through modulating the expression of several anti- and pro-apoptotic genes, deregulated cell-cycle, enhanced invasion & migration, upon HCMV infection or DUB overexpression in mammalian cell-lines, which was strongly correlated with inhibition of type I IFNs by HCMV-DUB. Conclusion: HCMV-deubiquitinase induces carcinogenic-properties through inhibiting Pattern-Recognition-Receptormediated type I IFNs via deubiquitination of important signaling molecules. Taken together, our results suggest that HCMV infection may promote oncogenesis by inhibiting host innate-immunity.