Journal of Clinical and Experimental Ophthalmology
Open Access
ISSN: 2155-9570
ISSN: 2155-9570
L.M.Balashova, Yu.D.Kuznetsova, L.S.Korobova, E.P.Kantarzhi, V.E.Muhin, E.A.Cherkashina, A.V. Popov, S.N.Bykovskaya and O Han Do
Pirogov Russian National Research Medical University Moscow, Russia
Morozov Russian Childrens Clinical Hospital, Russia
Childrens Clinical Hospital of Ministry of Health, Russia
NP International Scientific and Practical Center for Tissue Proliferation, Russia
Scientific Tracks Abstracts: J Clin Exp Ophthalmol
Purpose: The goal of the present work was initiated to study the role of regulatory T cells (Tregs) СD4+СD25+Foxp3+СD127low in autoimmune disorders retinopathy (RP) in various stages of prematurely born kids. Materials and Methods: The estimation of immunophenotype including Treg numbers in groups of mature donors (28), healthy mature and prematurely born kids with RP were compared. In total, 27 mature kids in age between 1,5 month to one year and 60 kids with RP from III+ to V active stages in age of 1,5 month to one year were studied in gestational ages from 25 to 32 weeks. Results: Comparison of the immunophenotype of healthy donor sand mature born kids revealed to decreasing of numbers of CD19+ B cells. Opposite, patients with RP have shown decreased numbers of CD3+, CD4+, CD4+CD25+FoxP3+CD127low T cells and elevated numbers of CD19+ B cells. There were no statistically reliable differences on immunological indicators when comparing children from 1 to 3 months, from 3.5 to 6 months and from 6.5 months to 1 year. Increased symptoms of the disease were followed by increase of B cells (CD19) numbers and statistically reliable decrease of regulatory T cells (?<0,05), decrease of CD4+ and natural killer cells (CD3-/CD16+CD56+). Evidently, expanded ex vivo autologous Tregs could be used for RP therapy. Соnclusion: Prematurely born kids with retinopathy demonstrated decreased numbers of peripheral regulatory T cells CD4+CD25+FoxP3+CD127low (Tregs). Tregs play a crutial role in the development of autoimmune diseases, and they can be a cause of complications in retinopathy. The results demonstrate reverse correlations between severe stages of retinopathy in premature born babies and low numbers of regulatory T cells CD4+CD25+FoxP3+CD127low as well as CD4+ and NK cells. Keywords: Retinopathy of prematurity, cellular immunity, T-regulators, B-cells, immunosuppression, auto-immunity.