Journal of Pharmaceutical Care & Health Systems
Open Access

Phytochemical analysis and antibacterial testing of Crinum macowanii bulbs

4^th African Pharma Congress

June 20-21, 2016 Cape Town, South Africa

Tendani Edith Sebola, N Niemann, V Mavumengwana and D T Ndinteh

University of Johannesburg, South Africa

Posters & Accepted Abstracts: J Pharma Care Health Sys

Abstract :

Crinum macowanii Baker is a medicinal plant native to southern Africa. It is commonly known as the Cape coast lily. The chemical makeup of the plant was investigated by phytochemical analysis using chromatography techniques such as Thin Layer Chromatography (TLC), Gravity Column Chromatography (GCC) and 2D Gas Chromatography (GCxGC TOF-MS). Phytochemical screening of plant crude extract showed the presence of tannins, reducing sugars, flavonoids, steroids, alkaloids, saponins and cardiac gylcosides. The results showed slight differences with a general trend that more phytochemicals were detected in autumn. The bulbs showed a strong result in the test for alkaloids, confirming that the plant is rich in this specific group of compounds. Antibacterial activity was noted for crude extracts against Mycobacterium smegmatis, Bacillus cereus and Staphylococcus epidermidis at a concentration of 1 mg/mL. In the disc diffusion experiments C. macowanii showed the highest antibacterial activity against Bacillus subtilis with a 12.67 mm zone of inhibition. The plant crude extract displayed no activity against Klebsiella pneumoniae, Proteus mirabilis and Proteus vulgaris for both disc diffusion and MIC, which are all Gramnegative bacteria. The only Gram-negative species that showed susceptibility to the bulb extract was Pseudomonas aeruginosa. From the GCxGC TOF-MS results, a number of compounds were detected such as oleic acid, 9,19-cycloergost-24(28)-en-3- ol, 4,14-dimethyl-,acetate (3�¡, 4� , 5� )-, trisphaeridine, dihydronormorphinone, xanthosine, flexinine and �±-sitosterol. These compounds have been reported to have biological applications such as anti-inflammatory, anti-microbial, anti-tumor, antiproliferative, antidiabetic, analgesic and lactation properties. These results imply that the active compounds found in bulbs are pharmaceutically useful for the production of drugs and this provides a basis for the justification of the traditional medicinal uses of the plant against the treatment of itchy rashes, boils, acne, backache and venereal disease.

Biography :

Email: 200905353@student.uj.ac.za